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This list contains a list of EC numbers for the third group, EC 3, hydrolases, placed in numerical order as determined by the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology.
EC 3.1: Acting on Ester Bonds
EC 3.1.1: Carboxylic Ester Hydrolases
- EC 3.1.1.1: carboxylesterase
- EC 3.1.1.2: arylesterase
- EC 3.1.1.3: triacylglycerol lipase
- EC 3.1.1.4: phospholipase A2
- EC 3.1.1.5: lysophospholipase
- EC 3.1.1.6: acetylesterase
- EC 3.1.1.7: acetylcholinesterase
- EC 3.1.1.8: cholinesterase
- EC 3.1.1.9: deleted
- EC 3.1.1.10: tropinesterase
- EC 3.1.1.11: pectinesterase
- EC 3.1.1.12: deleted
- EC 3.1.1.13: sterol esterase
- EC 3.1.1.14: chlorophyllase
- EC 3.1.1.15: L-arabinonolactonase
- EC 3.1.1.16: deleted, mixture of EC 5.3.3.4 and EC 3.1.1.24
- EC 3.1.1.17: gluconolactonase
- EC 3.1.1.18: deleted, included in EC 3.1.1.17
- EC 3.1.1.19: uronolactonase
- EC 3.1.1.20: tannase
- EC 3.1.1.21: retinyl-palmitate esterase
- EC 3.1.1.22: hydroxybutyrate-dimer hydrolase
- EC 3.1.1.23: acylglycerol lipase
- EC 3.1.1.24: 3-oxoadipate enol-lactonase
- EC 3.1.1.25: 1,4-lactonase
- EC 3.1.1.26: galactolipase
- EC 3.1.1.27: 4-pyridoxolactonase
- EC 3.1.1.28: acylcarnitine hydrolase
- EC 3.1.1.29: aminoacyl-tRNA hydrolase
- EC 3.1.1.30: D-arabinonolactonase
- EC 3.1.1.31: 6-phosphogluconolactonase
- EC 3.1.1.32: phospholipase A1
- EC 3.1.1.33: 6-acetylglucose deacetylase
- EC 3.1.1.34: lipoprotein lipase
- EC 3.1.1.35: dihydrocoumarin hydrolase
- EC 3.1.1.36: limonin-D-ring-lactonase
- EC 3.1.1.37: steroid-lactonase
- EC 3.1.1.38: triacetate-lactonase
- EC 3.1.1.39: actinomycin lactonase
- EC 3.1.1.40: orsellinate-depside hydrolase
- EC 3.1.1.41: cephalosporin-C deacetylase
- EC 3.1.1.42: chlorogenate hydrolase
- EC 3.1.1.43: a-amino-acid esterase
- EC 3.1.1.44: 4-methyloxaloacetate esterase
- EC 3.1.1.45: carboxymethylenebutenolidase
- EC 3.1.1.46: deoxylimonate A-ring-lactonase
- EC 3.1.1.47: 1-alkyl-2-acetylglycerophosphocholine esterase
- EC 3.1.1.48: fusarinine-C ornithinesterase
- EC 3.1.1.49: sinapine esterase
- EC 3.1.1.50: wax-ester hydrolase
- EC 3.1.1.51: phorbol-diester hydrolase
- EC 3.1.1.52: phosphatidylinositol deacylase
- EC 3.1.1.53: sialate O-acetylesterase
- EC 3.1.1.54: acetoxybutynylbithiophene deacetylase
- EC 3.1.1.55: acetylsalicylate deacetylase
- EC 3.1.1.56: methylumbelliferyl-acetate deacetylase
- EC 3.1.1.57: 2-pyrone-4,6-dicarboxylate lactonase
- EC 3.1.1.58: N-acetylgalactosaminoglycan deacetylase
- EC 3.1.1.59: juvenile-hormone esterase
- EC 3.1.1.60: bis(2-ethylhexyl)phthalate esterase
- EC 3.1.1.61: protein-glutamate methylesterase
- EC 3.1.1.62: now EC 3.5.1.47
- EC 3.1.1.63: 11-cis-retinyl-palmitate hydrolase
- EC 3.1.1.64: all-trans-retinyl-palmitate hydrolase
- EC 3.1.1.65: L-rhamnono-1,4-lactonase
- EC 3.1.1.66: 5-(3,4-diacetoxybut-1-ynyl)-2,2'-bithiophene deacetylase
- EC 3.1.1.67: fatty-acyl-ethyl-ester synthase
- EC 3.1.1.68: xylono-1,4-lactonase
- EC 3.1.1.69: now EC 3.5.1.89
- EC 3.1.1.70: cetraxate benzylesterase
- EC 3.1.1.71: acetylalkylglycerol acetylhydrolase
- EC 3.1.1.72: acetylxylan esterase
- EC 3.1.1.73: feruloyl esterase
- EC 3.1.1.74: cutinase
- EC 3.1.1.75: poly(3-hydroxybutyrate) depolymerase
- EC 3.1.1.76: poly(3-hydroxyoctanoate) depolymerase
- EC 3.1.1.77: acyloxyacyl hydrolase
- EC 3.1.1.78: polyneuridine-aldehyde esterase
- EC 3.1.1.79: hormone-sensitive lipase
EC 3.1.2: Thioester Hydrolases
- EC 3.1.2.1: acetyl-CoA hydrolase
- EC 3.1.2.2: palmitoyl-CoA hydrolase
- EC 3.1.2.3: succinyl-CoA hydrolase
- EC 3.1.2.4: 3-hydroxyisobutyryl-CoA hydrolase
- EC 3.1.2.5: hydroxymethylglutaryl-CoA hydrolase
- EC 3.1.2.6: hydroxyacylglutathione hydrolase
- EC 3.1.2.7: glutathione thiolesterase
- EC 3.1.2.8: deleted, included in EC 3.1.2.6
- EC 3.1.2.9: deleted
- EC 3.1.2.10: formyl-CoA hydrolase
- EC 3.1.2.11: acetoacetyl-CoA hydrolase
- EC 3.1.2.12: S-formylglutathione hydrolase
- EC 3.1.2.13: S-succinylglutathione hydrolase
- EC 3.1.2.14: oleoyl-(acyl-carrier-protein) hydrolase
- EC 3.1.2.15: ubiquitin thiolesterase
- EC 3.1.2.16: citrate lyase deacetylase
- EC 3.1.2.17: (S)-methylmalonyl-CoA hydrolase
- EC 3.1.2.18: ADP-dependent short-chain-acyl-CoA hydrolase
- EC 3.1.2.19: ADP-dependent medium-chain-acyl-CoA hydrolase
- EC 3.1.2.20: acyl-CoA hydrolase
- EC 3.1.2.21: dodecanoyl-(acyl-carrier protein) hydrolase
- EC 3.1.2.22: palmitoyl(protein) hydrolase
- EC 3.1.2.23: 4-hydroxybenzoyl-CoA thioesterase
- EC 3.1.2.24: transferred entry now EC 3.13.1.3
- EC 3.1.2.25: phenylacetyl-CoA hydrolase
- EC 3.1.2.26: bile-acid-CoA hydrolase
- EC 3.1.2.27: choloyl-CoA hydrolase
EC 3.1.3: Phosphoric Monoester Hydrolases
- EC 3.1.3.1: alkaline phosphatase
- EC 3.1.3.2: acid phosphatase
- EC 3.1.3.3: phosphoserine phosphatase
- EC 3.1.3.4: phosphatidate phosphatase
- EC 3.1.3.5: 5'-nucleotidase
- EC 3.1.3.6: 3'-nucleotidase
- EC 3.1.3.7: 3'(2'),5'-bisphosphate nucleotidase
- EC 3.1.3.8: 3-phytase
- EC 3.1.3.9: glucose-6-phosphatase
- EC 3.1.3.10: glucose-1-phosphatase
- EC 3.1.3.11: fructose-bisphosphatase
- EC 3.1.3.12: trehalose-phosphatase
- EC 3.1.3.13: bisphosphoglycerate phosphatase
- EC 3.1.3.14: methylphosphothioglycerate phosphatase
- EC 3.1.3.15: histidinol-phosphatase
- EC 3.1.3.16: phosphoprotein phosphatase
- EC 3.1.3.17: (phosphorylase) phosphatase
- EC 3.1.3.18: phosphoglycolate phosphatase
- EC 3.1.3.19: glycerol-2-phosphatase
- EC 3.1.3.20: phosphoglycerate phosphatase
- EC 3.1.3.21: glycerol-1-phosphatase
- EC 3.1.3.22: mannitol-1-phosphatase
- EC 3.1.3.23: sugar-phosphatase
- EC 3.1.3.24: sucrose-phosphatase
- EC 3.1.3.25: inositol-phosphate phosphatase
- EC 3.1.3.26: 4-phytase
- EC 3.1.3.27: phosphatidylglycerophosphatase
- EC 3.1.3.28: ADP-phosphoglycerate phosphatase
- EC 3.1.3.29: N-acylneuraminate-9-phosphatase
- EC 3.1.3.30: deleted, included in EC 3.1.3.31
- EC 3.1.3.31: nucleotidase
- EC 3.1.3.32: polynucleotide 3'-phosphatase
- EC 3.1.3.33: polynucleotide 5'-phosphatase
- EC 3.1.3.34: deoxynucleotide 3'-phosphatase
- EC 3.1.3.35: thymidylate 5'-phosphatase
- EC 3.1.3.36: phosphoinositide 5-phosphatase
- EC 3.1.3.37: sedoheptulose-bisphosphatase
- EC 3.1.3.38: 3-phosphoglycerate phosphatase
- EC 3.1.3.39: streptomycin-6-phosphatase
- EC 3.1.3.40: guanidinodeoxy-scyllo-inositol-4-phosphatase
- EC 3.1.3.41: 4-nitrophenylphosphatase
- EC 3.1.3.42: (glycogen-synthase-D) phosphatase
- EC 3.1.3.43: (pyruvate dehydrogenase (acetyl-transferring))-phosphatase
- EC 3.1.3.44: (acetyl-CoA carboxylase)-phosphatase
- EC 3.1.3.45: 3-deoxy-manno-octulosonate-8-phosphatase
- EC 3.1.3.46: fructose-2,6-bisphosphate 2-phosphatase
- EC 3.1.3.47: (hydroxymethylglutaryl-CoA reductase (NADPH))-phosphatase
- EC 3.1.3.48: protein-tyrosine-phosphatase
- EC 3.1.3.49: (pyruvate kinase)-phosphatase
- EC 3.1.3.50: sorbitol-6-phosphatase
- EC 3.1.3.51: dolichyl-phosphatase
- EC 3.1.3.52: (3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring))-phosphatase
- EC 3.1.3.53: (myosin-light-chain) phosphatase
- EC 3.1.3.54: fructose-2,6-bisphosphate 6-phosphatase
- EC 3.1.3.55: caldesmon-phosphatase
- EC 3.1.3.56: inositol-polyphosphate 5-phosphatase
- EC 3.1.3.57: inositol-1,4-bisphosphate 1-phosphatase
- EC 3.1.3.58: sugar-terminal-phosphatase
- EC 3.1.3.59: alkylacetylglycerophosphatase
- EC 3.1.3.60: phosphoenolpyruvate phosphatase
- EC 3.1.3.61: deleted
- EC 3.1.3.62: multiple inositol-polyphosphate phosphatase
- EC 3.1.3.63: 2-carboxy-D-arabinitol-1-phosphatase
- EC 3.1.3.64: phosphatidylinositol-3-phosphatase
- EC 3.1.3.65: now with EC 3.1.3.64
- EC 3.1.3.66: phosphatidylinositol-3,4-bisphosphate 4-phosphatase
- EC 3.1.3.67: phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase
- EC 3.1.3.68: 2-deoxyglucose-6-phosphatase
- EC 3.1.3.69: glucosylglycerol 3-phosphatase
- EC 3.1.3.70: mannosyl-3-phosphoglycerate phosphatase
- EC 3.1.3.71: 2-phosphosulfolactate phosphatase
- EC 3.1.3.72: 5-phytase
- EC 3.1.3.73: a-ribazole phosphatase
- EC 3.1.3.74: pyridoxal phosphatase
- EC 3.1.3.75: phosphoethanolamine/phosphocholine phosphatase
EC 3.1.4: Phosphoric Diester Hydrolases
- EC 3.1.4.1: phosphodiesterase I
- EC 3.1.4.2: glycerophosphocholine phosphodiesterase
- EC 3.1.4.3: lecithinase C
- EC 3.1.4.4: phospholipase D
- EC 3.1.4.5: now EC 3.1.21.1
- EC 3.1.4.6: now EC 3.1.22.1
- EC 3.1.4.7: now EC 3.1.31.1
- EC 3.1.4.8: now EC 3.1.27.3
- EC 3.1.4.9: now EC 3.1.30.2
- EC 3.1.4.10: now EC 4.6.1.13
- EC 3.1.4.11: phosphoinositide phospholipase C
- EC 3.1.4.12: sphingomyelin phosphodiesterase
- EC 3.1.4.13: serine-ethanolaminephosphate phosphodiesterase
- EC 3.1.4.14: (acyl-carrier-protein) phosphodiesterase
- EC 3.1.4.15: adenylyl-(glutamate—ammonia ligase) hydrolase
- EC 3.1.4.16: 2',3'-cyclic-nucleotide 2'-phosphodiesterase
- EC 3.1.4.17: 3',5'-cyclic-nucleotide phosphodiesterase
- EC 3.1.4.18: now EC 3.1.16.1
- EC 3.1.4.19: now EC 3.1.13.3
- EC 3.1.4.20: now EC 3.1.13.1
- EC 3.1.4.21: now EC 3.1.30.1
- EC 3.1.4.22: now EC 3.1.27.5
- EC 3.1.4.23: now EC 3.1.27.1
- EC 3.1.4.24: deleted
- EC 3.1.4.25: now EC 3.1.11.1
- EC 3.1.4.26: deleted
- EC 3.1.4.27: now EC 3.1.11.2
- EC 3.1.4.28: now EC 3.1.11.3
- EC 3.1.4.29: deleted
- EC 3.1.4.30: now EC 3.1.21.2
- EC 3.1.4.31: now EC 3.1.11.4
- EC 3.1.4.32: deleted
- EC 3.1.4.33: deleted
- EC 3.1.4.34: deleted
- EC 3.1.4.35: 3',5'-cyclic-GMP phosphodiesterase
- EC 3.1.4.36: now with EC 3.1.4.43
- EC 3.1.4.37: 2',3'-cyclic-nucleotide 3'-phosphodiesterase
- EC 3.1.4.38: glycerophosphocholine cholinephosphodiesterase
- EC 3.1.4.39: alkylglycerophosphoethanolamine phosphodiesterase
- EC 3.1.4.40: CMP-N-acylneuraminate phosphodiesterase
- EC 3.1.4.41: sphingomyelin phosphodiesterase D
- EC 3.1.4.42: glycerol-1,2-cyclic-phosphate 2-phosphodiesterase
- EC 3.1.4.43: glycerophosphoinositol inositolphosphodiesterase
- EC 3.1.4.44: glycerophosphoinositol glycerophosphodiesterase
- EC 3.1.4.45: N-acetylglucosamine-1-phosphodiester a-N-acetylglucosaminidase
- EC 3.1.4.46: glycerophosphodiester phosphodiesterase
- EC 3.1.4.47: now EC 4.6.1.14
- EC 3.1.4.48: dolichylphosphate-glucose phosphodiesterase
- EC 3.1.4.49: dolichylphosphate-mannose phosphodiesterase
- EC 3.1.4.50: glycosylphosphatidylinositol phospholipase D
- EC 3.1.4.51: glucose-1-phospho-D-mannosylglycoprotein phosphodiesterase
EC 3.1.5: Triphosphoric Monoester Hydrolases
EC 3.1.6: Sulfuric Ester Hydrolases
- EC 3.1.6.1: arylsulfatase
- EC 3.1.6.2: steryl-sulfatase
- EC 3.1.6.3: glycosulfatase
- EC 3.1.6.4: N-acetylgalactosamine-6-sulfatase
- EC 3.1.6.5: deleted
- EC 3.1.6.6: choline-sulfatase
- EC 3.1.6.7: cellulose-polysulfatase
- EC 3.1.6.8: cerebroside-sulfatase
- EC 3.1.6.9: chondro-4-sulfatase
- EC 3.1.6.10: chondro-6-sulfatase
- EC 3.1.6.11: disulfoglucosamine-6-sulfatase
- EC 3.1.6.12: N-acetylgalactosamine-4-sulfatase
- EC 3.1.6.13: iduronate-2-sulfatase
- EC 3.1.6.14: N-acetylglucosamine-6-sulfatase
- EC 3.1.6.15: N-sulfoglucosamine-3-sulfatase
- EC 3.1.6.16: monomethyl-sulfatase
- EC 3.1.6.17: D-lactate-2-sulfatase
- EC 3.1.6.18: glucuronate-2-sulfatase
EC 3.1.7: Diphosphoric Monoester Hydrolases
- EC 3.1.7.1: prenyl-diphosphatase
- EC 3.1.7.2: guanosine-3',5'-bis(diphosphate) 3'-diphosphatase
- EC 3.1.7.3: monoterpenyl-diphosphatase
EC 3.1.8: Phosphoric Triester Hydrolases
EC 3.1.11: Exodeoxyribonucleases Producing 5'-Phosphomonoesters
- EC 3.1.11.1: exodeoxyribonuclease I
- EC 3.1.11.2: exodeoxyribonuclease III
- EC 3.1.11.3: exodeoxyribonuclease (lambda-induced)
- EC 3.1.11.4: exodeoxyribonuclease (phage SP3-induced)
- EC 3.1.11.5: exodeoxyribonuclease V
- EC 3.1.11.6: exodeoxyribonuclease VII
EC 3.1.13: Exoribonucleases Producing 5'-Phosphomonoesters
- EC 3.1.13.1: exoribonuclease II
- EC 3.1.13.2: exoribonuclease H
- EC 3.1.13.3: oligonucleotidase
- EC 3.1.13.4: poly(A)-specific ribonuclease
EC 3.1.14: Exoribonucleases Producing 3'-Phosphomonoesters
EC 3.1.15: Exonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 5'-Phosphomonoesters
EC 3.1.16: Exonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 3'-Phosphomonoesters
EC 3.1.21: Endodeoxyribonucleases Producing 5'-Phosphomonoesters
- EC 3.1.21.1: deoxyribonuclease I
- EC 3.1.21.2: deoxyribonuclease IV (phage-T4-induced)
- EC 3.1.21.3: type I site-specific deoxyribonuclease
- EC 3.1.21.4: type II site-specific deoxyribonuclease
- EC 3.1.21.5: type III site-specific deoxyribonuclease
- EC 3.1.21.6: CC-preferring endodeoxyribonuclease
- EC 3.1.21.7: deoxyribonuclease V
EC 3.1.22: Endodeoxyribonucleases Producing 3'-Phosphomonoesters
- EC 3.1.22.1: deoxyribonuclease II
- EC 3.1.22.2: Aspergillus deoxyribonuclease K1
- EC 3.1.22.3: now EC 3.1.21.7
- EC 3.1.22.4: crossover junction endodeoxyribonuclease
- EC 3.1.22.5: deoxyribonuclease X
EC 3.1.23: and EC 3.1.24 now EC 3.1.21.3, EC 3.1.21.4 and EC 3.1.21.5
EC 3.1.25: Site-Specific Endodeoxyribonucleases Specific for Altered Bases
- EC 3.1.25.1: deoxyribonuclease (pyrimidine dimer)
- EC 3.1.25.2: now EC 4.2.99.18
EC 3.1.26: Endoribonucleases Producing 5'-Phosphomonoesters
- EC 3.1.26.1: Physarum polycephalum ribonuclease
- EC 3.1.26.2: ribonuclease alpha
- EC 3.1.26.3: ribonuclease III
- EC 3.1.26.4: calf thymus ribonuclease H
- EC 3.1.26.5: ribonuclease P
- EC 3.1.26.6: ribonuclease IV
- EC 3.1.26.7: ribonuclease P4
- EC 3.1.26.8: ribonuclease M5
- EC 3.1.26.9: ribonuclease (poly-(U)-specific)
- EC 3.1.26.10: ribonuclease IX
- EC 3.1.26.11: tRNase Z
EC 3.1.27: Endoribonucleases Producing 3'-Phosphomonoesters
- EC 3.1.27.1: ribonuclease T2
- EC 3.1.27.2: Bacillus subtilis ribonuclease
- EC 3.1.27.3: ribonuclease T1
- EC 3.1.27.4: ribonuclease U2
- EC 3.1.27.5: pancreatic ribonuclease
- EC 3.1.27.6: Enterobacter ribonuclease
- EC 3.1.27.7: ribonuclease F
- EC 3.1.27.8: ribonuclease V
- EC 3.1.27.9: tRNA-intron endonuclease
- EC 3.1.27.10: rRNA endonuclease
