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| Subtilisin | |
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 Crystal structure of Subtilisin |
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| protein type: | Serine protease |
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Subtilisin (serine endopeptidase) is a proteolytic enzyme initially obtained from Bacillus subtilis. It is secreted in large amounts from many Bacillus species.
The structure of subtilisin has been determined by X-ray crystallography. It is a 275 residue globular protein with several alpha-helices, and a large beta-sheet. It is structurally unrelated to the chymotrypsin-clan of serine proteases, but uses the same type of catalytic triad in the active site. This makes it the classic example of convergent evolution.
Charge-relay Site of Subtilisin
The active site features a charge-relay network involving Asp-32, His-64, and active site Ser-221 arranged in a catalytic triad. The charge-relay network functions as follows: The carboxylate side chain of Asp-32 hydrogen bonds to a nitrogen-bonded proton on His-64's imidazole ring. This is possible because Asp is negatively charged at physiological pH. The other nitrogen on His-64 hydrogen bonds to the O-H proton of Ser-221. This last interaction results in charge-separation of O-H, with the oxygen atom being more nucleophilic. This allows the oxygen atom of Ser-221 to attack incoming substrates (ie. peptide bonds), assisted by a neighboring carboxyamide side chain of Asn-155.
Even though Asp-32, His-64, and Ser-221 are sequentially far apart, they converge in the 3D structure to form the active site.
To summarize the interactions described above, Ser-221 acts as a nucleophile and cleaves peptide bonds with its partially negative oxygen atom. This is possible due to the nature of the charge-relay site of subtilisin.
References
Deber, C.M. (Lecturer). (2006, Sep. 29). BCH210H1F. [Lecture]. Toronto: University of Toronto.
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